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Source: | Rice Grain(Oryza Sativa) | Purity: | Greater Than 99% |
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Production Type: | Recombinant Protein | PH: | 6.4-7.4 |
EINECS No.: | 274-272-6 | Endotoxin: | Less Than 0.125EU/mg |
Highlight: | High Purity rHSA Cell Culture,66.5KD Recombinant Human Serum albumin,Recombinant HSA Cell Culture Oryzogen |
High Purity Oryzogen Human Serum albumin HSA Cell Culture 66.5KD Molecular Weight
Quick detail
Physiological and Biochemical Properties of rHSA and pHSA
Char. | pHSA | rHSA |
Amino Acids Sequence | Same | Same |
N-terminus | DAHKSEV | DAHKSEV |
C-terminus | KLVAASQAALGL | KLVAASQAALGL |
Glycosylation | No | No |
Molecular Mass(MALDI) | 66.554KD | 66.550KD |
PI | 4.8 | 4.8 |
Drug Binding | Similar | Similar |
Thermal Stability | M/P 65℃ | M/P 65℃ |
Lipid Conjugation | Identical | Identical |
Crystal Structure | Identical | Identical |
Description
The quality control of stem cell culture process is very strict, have ing high requirements of various indexes such as bacteria, endotoxin and PH. The rHSA facility is compliance with the GMP standard, with many advantages such as animal component free, high purity, low endotoxin and so on. It can ultimatedly meet the level of stem cell culture.
rHSA is an important supplement in cell culture medium. It is an important carrier of of hormone, lipid and nutrition in the cell culture media. It plays an important role in cell growth. rHSA in the cell culture medium can provide the nutrions needed for cell growth, increase the viscosity of the medium, provide a mechanical protection for cells. Thereby, it can achieve the purpose of accelarating cells growth.
Recombinant human serum albumin (OsrHSA) has good efficacy, such as OsrHSA has the same second and tertiary structure, physiological and biochemical properties with HSA from plasma. In addition, compared with HSA from plasma, OsrHSA has a lower immune source stimulation, and plays a better role in accelerating cell growth and metabolism, because OsrHSA is animal component free, which can avoid the prions or virus contaminants.
In the CIK serum free cell culture medium, the recombinant human serum albumin can promote CIK cell growth significantly.
In the vero cell culture, different batch of recombinant HSA have the same performantce with 10% FBS.
Advantages
1. Healthgen Biotech recombinant human serum albumin is free of animal origin, which effectively avoid any potential risk of animal bacteria, prions, virus and contagious protein disease.
2. Wuhan Healthgen Biotechnology Corp. has been committed in research of protein purification and meanwile our technology has been patented by the US Patent and Trademark Office on May 15th 2015. "A method for isolating and purifying recombinant human serum albumin from transgenic rice grain". The patent number is US9, 023,990. The purity of our rHSA is very high. Now, our rHSA purity can be as high as 99%.
3. The rice endosperm specific expression platform is easy to be large-scale. The rice endosperm has the protein body, which can keeps proteins stability and bioactivity. At present, the production capacity is almost 1000 kg / year.
Supplemental Description
Healthgen Biotech has strong technical force and advanced and complete protein expression and purification technology development and large-scale production facilities. Self-developed rice endosperm cell expression platform (OryzHiExp), which is domestic initiative and the international leader, has such advantages: high expression level, safety, scale-up easily, simple production process and environmental protection etc. After 10 years of unremitting efforts, Healthgen Biotech has established recombinant protein expression and purification technology platform to customize recombinant protein or peptide expression and purification process service for pharmaceuticals, health products, scientific research and industrial reagents.
Contact Person: Ms. Abby
Tel: +86-15071115810
Fax: 86-27-59403933-59416006