Promote Antibody Production Recombinant Human Albumin Rice Grain Derived Molecular Weight 66.5 KDa

Recombinant human albumin powder promote antibody production

Human serum albumin (HSA) is the major protein component of human plasma, which consists of three domains composed of 585 amino acidsand is a heart-shaped molecule with molecular weight of 66.5 kDa. HSA is synthelized in the liver and is mainly responsible for maintaining normal osmolarity in the blood circulation system, a carrier for numerous small molecules. HSA is widely used as an excipient for vaccines or therapeutic protein drugs, and as a cell culture medium supplement in the production of vaccines and pharmaceuticals.

Serum-derived albumin has for a long time been used in cell culture media. Currently, many commercial companies are developing animal component free media that are based on safety considerations for the production. Healthgen Biotech’s recombinant human albumin (OsrHSA) is derived from rice endosperm, which is animal component free, can avoid the prions or virus contaminants.

Physiological and Biochemical Properties of rHSA and pHSA

Recombinant human albumin is used as a kind of necessary nutrient material in the cell culture and metabolism process. In addition, as a carrier protein, OsrHSA provide necessary elements for cell metabolism synthesis and transportation. In SP 2/0 cell culture, the OsrHSA can promote the cell growth and antibody secrection. Compared with the SP 2/0 in the FBS, the OsrHSA has the same result, which indicated that the OsrHSA has effective in the serum free medium to promote antibody secrection.

Using the recombinant human albumin in the cell culture media to produce the antibodies, there are such advantages:

• Improvde the target proteins expression level and make the cell products easily purified;
• Animal component free, can avoid the prions or virus contaminants;
• Avoid the serum quality changes from different batches;
• Well defined cell culture medium component;
• Improve the repeatability of cell culture and experiment results;
• Avoid the toxic effects and serum-derived contamination from serum to cells;
• Avoid the impact of serum components in the antibody study.