Large Scale Production Recombinant Human Albumin 65 KD Molecular Weight

Large scale production of recombinant human albumin powder

HSA has been widely used in pharmaceutical applications and cell cultures for many decades. Currently, HSA is only obtained from human plasma. Healthgen Biotech successfully expressed recombinant human albumin (OsrHSA) in rice seeds and developed a cost-effective purification protocol for large-scale OsrHSA production. OsrHSA is determined biochemically, structurally, functionally, and immunologically equivalent to pHSA, demonstrating that rice endosperm is a safe and promising alternate system for the large-scale production of OsrHSA.

Physiological and Biochemical Properties of OsrHSA and pHSA

Rice seeds as bioreactor show great promise as a flexible system for the production and processing of recombinant human albumin (OsrHSA) with intact biochemical features on a large scale. The rice endosperm specific expression platform is easy to be large-scale. The rice endosperm has the protein body, which can keeps proteins stability and bioactivity. At present, the production capacity is almost 1000 kg / year.

In recent years, there has been an increased demand for animal-free components to replace serum- or animal-originated supplements to eliminate the risk of passage of prion-related diseases to humans. Use of the rice seed bioreactor could provide an economical and safe approach for the production of non–animal-derived compounds, which will facilitate development of products from cell cultures. Supplementing 1 g/L of OsrHSA in serum-reduced media results in cell growth comparable to that seen using 10% FBS, indicating that OsrHSA can reduce the use of FBS in cell culture. Use of the rice seed bioreactor could provide an economical and safe approach for the production of non–animal-derived compounds, which will facilitate development of products from cell cultures.