Yeast Derived Recombinant Proteinase K High Activity 40 U / mgP

Description

Proteinase K is a serine protease, which is a major kind of proteases produced by Tritirachiurn album Limber. Because microorganisms that can synthesize this protease can grow in an environment with kerantin as the sole carbon and nitrogen source, so it is named “proteinase K”. Proteinase K has extremely high enzymatic activity and broad substrate specificity. It can preferentially decompose ester bonds and peptide bonds adjacent to the C-terminus of hydrophobic amino acids, sulfur-containing amino acids, and aromatic amino acids. It is often used to degrade proteins to produce short peptides. It has the characteristics of the typical catalytic triplet Asp39-His69-Ser224 of serine proteases and has two Ca binding sites around the active center to increase its stability and maintain high enzymatic activity under a wider range of conditions. Proteinase K can inactivate or degrade proteins. Using this characteristic, proteinase K has important applications in the fields of nucleic acid purification, silk, medicine, food and brewing.

Currently commercially available proteinase K is mainly isolated and purified from Tritirachiurn album Limber by fermentation. As long as the medium contains protein, Tritirachiurn album Limber will be induced to produce proteinase K, so the fermentation medium generally needs to add BSA, milk powder or soybean meal as a nitrogen source. After the growth enters the stationary phase, the bacteria begin to secrete and express proteinase K. However, the biosynthesis of proteinase K during this process is inhibited by glucose and amino acids in the medium. In addition, the production of proteinase K by fermentation is difficult to scale up, so the production capacity of commercial proteinase K is generally relatively low.

The recombinant proteinase K(rProK) of Healthgen Biotech is derived from recombinant expression of genetically engineered yeast, and purified by chromatography process with calcium and glycerin as protective agents, which perfect solves the problems of activity inhibition and low production capacity in the fermentation production process of natural proteinase K. rProK has high activity and high capacity, as well as other advantages, such as:

1. High activity greater than 30U/mg and specific activity greater than 40 U/mgP.
2. Cost-effective due to the high production capacity more than 80kg/month.
3. Excellent stability within and between batches as well as excellent thermal stability, which has complete stability data of the room temperature and accelerated test.
4. Stable supply: high capacity with 80kg per month and can meet bulk demands.
5. Easy to store: store at 2-8 degrees for more than 36 months.
6. DNase and RNase free
7. Can extract ultra-micro amounts of nucleic acid which has been verified by many of our customers and the results are good.

• Other Names: Recombinant ProK, rPK, Proteinase K, rProK, PK
• Certificate: ISO9001:2015 certificate
• Molecular weight: 29kD
• Package size: 1g, 10g, 50g, 100g

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