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| Other Names: | Recombinant ProK, RPK, Proteinase K, RProK, PK | CAS No.: | 39450-01-6 |
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| Model Number: | HYC032R03 | Purity: | Greater Than 95% As Determined By SDS-PAGE |
| Product Type: | Recombinant Protein | Certificate: | ISO9001:2015 Certificate |
| Highlight: | proteinase k lyophilized powder,Recombinant proteinase k lyophilized powder,HYC032R03 |
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High quality research reagent, recombinant proteinase K lyophilized powder
Description
Proteinase K is a broad-spectrum serine protease originally isolated from fungus Engyodontium album (Tritirachium album). Proteinase K was named “proteinase K” for its ability to digest keratin. Structural and molecular biology studies suggest that the enzyme belongs to the subtilisin family characterized with a catalytic triad (Asp39-His69-Ser224) in the active site. It is stable (and functional) up to 65 degrees C and will function in temperatures down to 25 degrees C (room temperature).
Healthegn Biotech express and purify the recombinant proteinase K (rProK) from yeast (Pichia Pastoris) based on the optimized gene of Engyodontium album by site-directed mutation. The rProK possesses a high specific activity which remains stable over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Purified by chromatography process, the rProK has a high purity more than 95%. The rProK can be used in a variety of molecular biology, cell biology and other related experiments, such as genomic DNA extraction, enzyme digestion and removal, cell permeability and so on.
Product Information
Cat No. | HYC032R03 |
CAS No. | 39450-01-6 |
E.C. | 3.4.21.64 |
Synonyms | Peptidase K, endoproteinase K, endopeptidase K |
Source | From yeast with cloned gene encoding genetically engineered Engyodontium album (Tritirachium album) endolytic protease |
Physical Appearance | Lyophilized powder |
Molecular Mass | 29.3 kD |
pI | 8.9 |
pH Range | 4.5-12.0 (optimum pH range 7.5-11.5) |
Purity | ≥95% |
Specific Activity | ≥30 U/mg |
Unit Definition | One unit is defined as the enzyme activity that produces 1 μmol of tyrosine per minute from Bovine Hemoglobin at 37°C, pH 7.5. |
Storage Temperature | Recommended at 2~8 °C. |
DNase | None detected. |
RNase | None detected. |
Shelf Life | Two years |
Advantages of Our Recombinant Proteinase K
Contact Person: Ms. Abby
Tel: +86-15071115810
Fax: 86-27-59403933-59416006
| Factory Address:268 Shendun 5th Road, East Lake High-Tech Development Zone, Wuhan, China 430020 | |
| Sales office:268 Shendun 5th Road, East Lake High-Tech Development Zone, Wuhan, China 430020 | |
| +86-27-59301891-8015 | |
| caojing@oryzogen.com | |
