Recombinant Proteinase K For COVID-19 Nucleic Acid Detection CAS 39450-01-6

Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification ideal for these applications because proteinase K is able to break down proteins and inactivate DNases and RNases that would otherwise degrade a desired sample of DNA or RNA. The proteinase K possesses a high specific activity which remains stable over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Proteinase K was used widely in COVID-19 nucleic acid automatic extraction, which help to isolate of highly native, undamaged nucleic acid and improve the detection accuracy.

Healthegn Biotech express and purify the recombinant proteinase K (rProK) from yeast (Pichia Pastoris) based on the optimized gene of Engyodontium album by site-directed mutation. The rProK possesses a high specific activity which remains stable over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Purified by chromatography process, the rProK has a high purity more than 95%, which is also completely free of DNase and RNase activities.

Source: Yeast

Molecular weight: 29kD

Purity: More than 95%

Bioactivity: ≥30U/mg detected using hemoglobin as substrate

E.C. No. 3.4.21.64

DNase: Non-detection

RNase: Non-detection

Storage Conditions: Store at 4 °C

Shelf Life: 24 months

Form: lyophilised powder and liquid (20mg/ml)