DNA & RNA Purification Enzyme Proteinase K Bulk Supply 39450-01-6

Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification ideal for these applications because proteinase K is able to break down proteins and inactivate DNases and RNases that would otherwise degrade a desired sample of DNA or RNA. Proteinase K is cation. It is highly-suited to this application since the enzyme is active in the presence of chemicals that denature proteins, such as SDS and urea, chelating agents such as EDTA, sulfhydryl reagents, as well as trypsin or chymotrypsin inhibitors. It is used for the destruction of proteins in cell lysates (tissue, cell culture cells) and for the release of nucleic acids, since it very effectively inactivates DNases and RNases. Some examples for applications: Proteinase K is very useful in the isolation of highly native, undamaged DNA or RNA, since most microbial or mammalian DNases and RNases are rapidly inactivated by the enzyme, particularly in the presence of 0.5 – 1% SDS.

The advantages of recombinant proteinase K from Healthgen Biotech:

• High activity - broad-spectrum serine protease
• Bulk quantity supply - More than 20kg production capacity every month
• Active under denaturing conditions - useful for a variety of applications
• Stable - at high temperatures
• Molecular biology grade - useful for general digestion of proteins
• Two formats - lyophilized powder and liquid

The recombinant proteinase K was used to purify the DNA, and the result showed that the DNA was undemaged and the rProK was also completely free of DNase.