PCR Grade Recombinant Proteinase K Bulk Quantity Supply 39450 01 6

Recombinant Proteinase K bulk quantity supply PCR grade

Description

Proteinase K, a broad-spectrum serine protease, firstly was extracted and purified from Engyodontium album. Because it can degrade Keratin, so named proteinase K. The crystallization and molecular structure of Proteinase K indicate it belongs to subtilisin family, whose active site has a characteristic catalytic triple amino acid Asp39-His69-Ser224. Proteinase K has no significant substrate specificity, and main cleavage site is the peptide bond at the carboxyl end of the hydrophobic amino acid such as aliphatic or aromatic.

Healthgen Biotech recombinant Proteinase K is the same as the natural protease originally isolated from Tritirachium album, which has same amino acid sequence (molecular weight) and molecular structure. However, the recombinant proteinase K (rProK) is more pure than the natural enzyme, because according to current quality control procedures, the rProK does not contain DNA or RNA, so it is very suitable for separating PCR and RT-PCR templates.

Application

• This PCR Grade recombinant proteinase K (rProK) is very effective against natural proteins, so it can quickly inactivate endogenous nucleases such as RNase and DNase. This feature makes proteinase K particularly suitable for separating natural RNA and DNA from tissues or cells.
• The rProK promotes cell lysis by activating bacterial autolysis factors.
• The rProK analyzes the membrane structure by modifying the proteins and glycoproteins on the cell surface.
• The rProK is particularly suitable for separating nucleic acids for PCR.
• The rProK can be used to remove cell debris during colony transfer preparations and to process tissue sections to ensure effective probe infiltration during in situ hybridization.

Healthgen Biotech recombinant Proteinase K is extensively purified to give highly active formulation free of any detectable nuclease activities. It is fully active up to 56°C and under denaturing conditions (e.g. in the presence of urea and/or SDS), what makes it ideal for DNA extraction application.