CAS No 39450 – 01 - 6 Recombinant Proteinase Kinase Digest A Variety Of Proteins

Description

Proteinase K is an important serine protease secreted by Tritirachium album Limber, containing the typical catalytic triad Asp39-His69-Ser224 characteristic of serine proteases, and has a strong ability to degrade natural proteins. It consists of 279 amino acids, containing 2 disulfide bonds and 1 free cysteine, which makes proteinase K maintain high stability in high temperature and extreme pH environment. Proteinase K contains two Ca2+ binding sites, which also increase the thermal stability as well as catalytic activity.

Proteinase K can be used for the digestion of a variety of proteins, including the preparation of chromosomal DNA for pulsed electrophoresis, western blotting, and the removal of nucleases in DNA and RNA preparations. The application of proteinase K in nucleic acid extraction involves many fields such as food-borne disease prevention, agricultural pest control, forensic gene identification and comparison, and molecular biology experimental research. Application objects include prokaryotes, human blood samples and tissues, animal and plant tissues, parasites, microorganisms, etc.

The currently commercially available proteinase K is mainly secreted by Tritirachium album Limber, which grows slowly and is difficult to cultivate at high density, and the yield of proteinase K is low. In addition, Tritirachium album Limber also secretes other proteases, which increases the difficulty of downstream separation and purification.

Healthgen Biotech’s recombinant proteinase K (rProK) which is derived from yeast and purified by chromatography process with calcium and glycerin as protective agents, provides an optimized solution the problems of insufficient production and low purity of natural proteinase K. The detail information of rProK is as follow.

CAS No.: 39450-01-6

Other Names: Recombinant ProK, rPK, Proteinase K, rProK, PK

Place of Origin: Wuhan, China (Mainland)

Brand Name: Oryzogen

Model Number: HYC032R03

Purity: Greater than 95% as determined by SDS-PAGE

Source: Yeast (Pichia pastoris)

Gene Source: Engyodontium album

Physical Appearance: Off- White Loose lyophilized powder

Formulation: Lyophilized powder

Activity: More than 30U/mg

Specific activity: More than 40U/mgP

Storage and Handling: Stored at temperatures 2-8℃ for 36 months

Product Type: Recombinant protein

Certificate: ISO9001:2015 certificate

Molecular weight: 29kD

Package size: 1g, 10g, 50g, 100g

Supplemental Description

1. The chairman of Healthgen Biotech Professor Daichang.Yang is one of China's chief scientists. Professor Yang has rechieved the “Thousand Talents program” of the National Central Organization Department, "Hundred Talents Program" of the Organization Department of Hubei Province and Top 10 "Venture Eagle" entrepreneurs.
2. With years of effort, Healthgen Biotech has developed the State-of-the-Art platform called OryzHiExp, having high capacity to express no animal origin proteins and peptides in the rice endosperm cells.
3.  Healthgen Biotech quality control systerm has been certified by ISO 9001:2015 and ensure standardized process as well as qualified products.