ISO 9001 2015 Certificate Recombinant Proteinase K Extracting Nucleic Acids

Description

Proteinase K (EC 3.4.21.14) is a serine protease related to subtilisin, firstly was extracted and purified from Engyodontium album. It is named proteinase K because of its ability to degrade keratin. Proteinase K consists of a peptide chain containing 279 amino acid residues with a relative molecular mass of 28,930 Daltons. It is a highly active broad-spectrum protease, which can still maintain high enzymatic activity at 65°C and in the presence of EDTA, urea and detergents such as 0.5% sodium dodecyl sulfonate (SDS) solution or 1% Triton X-100, and has great advantages in extracting high molecular weight nucleic acids.

The separation and purification technology of nucleic acid is a basic technology of biochemistry and molecular biology. Nucleic acids are always combined with various proteins in cells. The separation of nucleic acids mainly refers to the separation of nucleic acids from biological macromolecules such as proteins, polysaccharides, and fats. When isolating nucleic acid, the contamination of other molecules should be excluded as much as possible to ensure the purity of the nucleic acid sample. Proteinase K has important applications in the separation and purification of nucleic acids, which can degrade proteins bound to nucleic acids and promote the separation of nucleic acids.

Healtgen Biotech has extracted recombinant proteinase K (rProK) from Yeast by genetic engineering technology, and purified the rProK by chromatography process with calcium and glycerin as protective agents. Compared to commercial natural, rProK has higher purity and activity. Moreover, Healthgen Biotech’s advanced production process breaks through the production limit of natural and other source of recombinant proteinase K, which makes its high capacity reaching 80kg/month, and then can better meet the huge needs of today's nucleic acid detection manufacturers.

Detail

CAS No.: 39450-01-6

Other Names: Recombinant ProK, rPK, Proteinase K, rProK, PK

Place of Origin: Wuhan, China (Mainland)

Brand Name: Oryzogen

Model Number: HYC032R03

Purity: Greater than 95% as determined by SDS-PAGE

Source: Yeast (Pichia pastoris)

Gene Source: Engyodontium album

Physical Appearance: Off- White Loose lyophilized powder

Formulation: Lyophilized powder

Activity: More than 30U/mg

Specific activity: More than 40U/mgP

Storage and Handling: Stored at temperatures 2-8℃ for 36 months

Product Type: Recombinant protein

Certificate: ISO9001:2015 certificate

Molecular weight: 29kD

Package size: 1g, 10g, 50g, 100g