80kg/month Recombinant Proteinase K For RNA Extraction

Description

Proteinase K (EC 3.4.21.14) is a serine protease related to subtilisin, firstly was extracted and purified from Engyodontium album. It is named proteinase K because of its ability to degrade keratin. Proteinase K consists of a peptide chain containing 277 amino acid residues with a relative molecular mass of 28,930 Daltons. It is a highly active broad-spectrum protease, which can still maintain high enzymatic activity in denaturant urea or sodium dodecyl sulfonate (SDS) solution, so it is widely used for DNA or RNA extraction in various biological materials.

In the process of RNA extraction, proteinase K is always used for RNase inhibition and degradation. RNA quality is critical to the success of experiments such as Northern blotting and hybridization analysis, cDNA synthesis, and in vitro translation. RNA is extremely unstable and easily degraded by ubiquitous RNases, therefore, using proteinase K to create an RNase-free environment and strictly preventing RNase contamination is the key to successful RNA extraction, especially in Covid-19 virus RNA extraction.

Recombinant Proteinase K (rProK), derived from yeast, is purified by chromatography process with calcium and glycerin as protective agents without RNase and DNase. Compared to natural proteinase K, rProK has the same structure and function as natural proteinase K while it has higher purity greater than 95% and higher activity greater than 30 U/mg, as well as higher specific activity greater than 40U/mgP. Compared to other source of recombinant proteinase K, Healthgen Biotech’s rProK also has a series of advantages, such as:

1. High activity greater than 40U/mgP.
2. Cost-effective due to the high production capacity more than 80kg/month.
3. Excellent stability within and between batches as well as excellent thermal stability, which has complete stability data of the room temperature and accelerated test.
4. Stable supply: high capacity with 80kg per month and can meet bulk demands.
5. Easy to store: store at 2-8 degrees for more than 36 months.
6. DNase and RNase free
7. Can extract ultra-micro amounts of nucleic acid which has been verified by many of our customers and the results are good.

Detail

E.C. No.: 3.4.21.64

Other Names: Recombinant ProK, rPK, Proteinase K, rProK, PK

Model Number: HYC032R03

Purity: Greater than 95% as determined by SDS-PAGE

Activity: More than 30U/mg

Specific activity: ≥40 U/mgP

Source: Yeast (Pichia pastoris)

Storage and Handling: Stored at temperatures 2-8℃ for 36 months

Certificate: ISO9001:2015 certificate

Package size: 1g, 10g, 50g, 100g