ProK Proteinase K Cas Number 39450-01-6 Molecular Biology Grade From Direct Manufacturer

Proteinase K is a broad spectrum endopeptidase with very high specific activity. It is widely used for

digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising integrity of isolated DNA or RNA. Proteinase K is active under wide range of reaction conditions, including elevated temperatures and presence of SDS, urea, EDTA and trypsin.

Healthegn Biotech express and purify the recombinant proteinase K (rProK) from yeast (Pichia Pastoris) based on the optimized gene of Engyodontium album by site-directed mutation. The rProK possesses a high specific activity which remains stable over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Purified by chromatography process, the rProK has a high purity more than 95%, which is also completely free of DNase and RNase activities. As the manufacturer, Healthgen Biotech has more than 20kg production capacity every month, can provide stable supply and favorable price.

Appearance: White lyophilized powder

Solubility in water: ≥ 20 mg/ml

Activity: ≥ 30 U/mg lyophilized powder

               ≥ 40 U/mg protein

One unit of recombinant Proteinase K hydrolyzes urea-denaturated hemoglobin producing color equivalent of 1 μmol tyrosine per 1 min at 37°C and pH = 7.5 (Folin & Ciocalteu’s method), 1 U = 1 mAnsonU.

Protein content: ≥ 90% (Protein content is determined by kjeldahl determination)

Stock solution preparation: 50 mM Tris-HCl, pH = 7.8, 3 mM CaCl2 for immediate use, or 50 mM

Tris-HCl, pH = 7.8, 3 mM CaCl2 , 50% glycerol for long-term storage at -20˚C.

Formulation: The recombinant Proteinase-K was lyophilized without any additives

The advantages of recombinant proteinase K from Healthgen Biotech:

Wuhan Healthgen Biotechnology Corp. is a private company founded in 2006. We specialize in molecular pharmaceutical technology research and high-tech innovation-oriented product development, which is developing, manufacturing and marketing a series of recombinant proteins and growth factors. We have 400 square meters of research center, in which 30% of the research personnels have doctoral degree and major in different directions of the research and development. Healthgen has strong capability in developing a series of biological products.

Healthgen Biotech quality control systerm has been certified by ISO 9001:2015 and ensure standardized process as well as qualified products.