Engyodontium Album Proteinase K Recombinant High Purity 50–100 µg/ML

Proteinase K, a broad-spectrum serine protease, firstly was extracted and purified from Engyodontium album. Because it can degrade Keratin, so named proteinase K. The crystallization and molecular structure of Proteinase K indicate it belongs to subtilisin family, whose active site has a characteristic catalytic triple amino acid Asp39-His69-Ser224. Proteinase K has no significant substrate specificity, and it cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids and is useful for general digestion of protein in biological samples.

Healthegn Biotech express and purify the recombinant proteinase K (rProK) from yeast (Pichia Pastoris) based on the optimized gene of Engyodontium album by site-directed mutation. The rProK possesses a high specific activity which remains stable over a wide range of temperatures and pH values with substantially increased activity at higher temperature. The stability of rProK in urea and SDS and its ability to digest native proteins make it useful for a variety of applications, including preparation of chromosomal DNA for pulsed-field gel electrophoresis, protein fingerprinting and removal of nucleases from preparations of DNA and RNA. A typical working concentration for Proteinase K is 50–100µg/ml.

Molecular Weight: 29 kDa.

Source: Yeast

Activity: ≥30U/mg (One unit of recombinant Proteinase K hydrolyzes urea-denaturated hemoglobin producing color equivalent of 1 μmol tyrosine per 1 min at 37°C and pH = 7.5 (Folin & Ciocalteu’s method)

Purity: more than 95%, SDS-PAGE

E.C. No.: 3.4.21.64

DNase: Non-detection

RNase: Non-detection

Application Features

• Isolation of plasmid and genomic DNA
• Isolation of RNA
• Inactivation of RNases, DNases and enzymes in reactions
• Removal of enzymes from DNA to improve cloning efficiency
• PCR purification

Wuhan Healthgen Biotechnology Corp. is a private company founded in 2006. We specialize in molecular pharmaceutical technology research and high-tech innovation-oriented product development, which is developing, manufacturing and marketing a series of recombinant proteins and growth factors. Healthgen Biotech quality control systerm has been certified by ISO 9001:2015 and ensure standardized process as well as qualified products.