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| Other Names: | Recombinant ProK, RPK, Proteinase K, RProK, PK | CAS No.: | 39450-01-6 |
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| Product Type: | Recombinant Protein | Certificate: | ISO9001:2015 Certificate |
| Molecular Weight: | 29kD | Package Size: | 1g, 10g, 50g, 100g |
| Highlight: | Recombinant Proteinase K powder,Proteinase K powder 100g,Proteinase K Size 100g |
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Recombinant Proteinase K powder Molecular Biology Grade high purity
Description
The Proteinase K, a member of the Peptidase family S8, is a broad-spectrum serine protease. Proteinase K is capable of digesting hair (keratin), henceforth, the name "Proteinase K". Proteinase K is activated by calcium, the enzyme digests proteins especially after hydrophobic amino acids (aliphatic, aromatic and other hydrophobic amino acids). Proteinase K is frequently utilized in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases which may otherwise degrade the DNA or RNA during purification. Proteinase K is utilized for the destruction of proteins in cell lysates (tissue, cell culture cells) and for the release of nucleic acids, given that it quite effectively inactivates DNases and RNases.
Healthegn Biotech express and purify the recombinant proteinase K (rProK) from yeast (Pichia Pastoris) based on the optimized gene of Engyodontium album by site-directed mutation. The rProK possesses a high specific activity which remains stable over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Purified by chromatography process, the rProK has a high purity more than 95%, which is also completely free of DNase and RNase activities.
Grade: Molecular Biology Grade
Purity: Greater than 95%.
Biological activity: more than 30U/mg (one unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 µmol tyrosine per min at 37°C, pH 7.5 (color by Folin-Ciocalteu reagent)).
Formulation: The recombinant Proteinase-K was lyophilized without any additives
Reconstitution: It is recommended to reconstitute the lyophilized Proteinase K powder in sterile water or 20mM Tris-HCl (pH 7.4~8.0), 1mM CaCl2, 50% glycerol at 20mg/ml, which can then be further diluted to other aqueous solutions.
Applications
Contact Person: Ms. Abby
Tel: +86-15071115810
Fax: 86-27-59403933-59416006
| Factory Address:268 Shendun 5th Road, East Lake High-Tech Development Zone, Wuhan, China 430020 | |
| Sales office:268 Shendun 5th Road, East Lake High-Tech Development Zone, Wuhan, China 430020 | |
| +86-27-59301891-8015 | |
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