Rice Grain Recombinant Fibronectin For Stem Cell Culture CAS 86088-83-7

Recombinant fibronectin for stem cell culture

Description

Fibronectin, an abundant and ubiquitous protein of the extracellular matrix, forms a fibrillar network by interacting directly with cell surface receptors. It is synthesized by a variety of different cells and is secreted as a disulfide-bonded dimer with 220 kDa subunits. FN is a mosaic protein composed of three types of repeating modular consensus amino acid sequences. Functional domains of FN mediate interactions with cell surface receptors and a number of other macromolecules including fibrin, fibrinogen, collagen, heparin, immune complexes, and fibronectin itself. So FN is known to play a key role in cell adhesion, spreading, migration, differentiation and proliferation via interactions with transmembrane integrin receptors.

Stemcells are defined by three essential features, as they are i) undifferentiated cells able to give rise both to ii) differentiated daughter cells and iii) cells that retain their stemness by self-renewal. The FN can be an player in stem cell culture, since it can directly or indirectly modulate the maintenance, proliferation, self-renewal and differentiation of stem cells, which is a key component of stem cell niches and is involved in various aspects of stem cell behavior, thus having a major impact on tissue homeostasis and regeneration under physiological and pathological conditions. Numerous studies has demonstrated that FN works very important role in the stem cell culture, like FN result in murine HSCs expansion of primitive stem cells and improvement in the marrow engraftibility.

Currently, the FN is purified from the plasma, which however is limited by the availability of supply. In addition, due to shortage of plasma and low expression levels of recombinant fibronectin in conventional gene expression systems, Healthgen Biotech is focusing on developing, manufacturing and marketing a series of environmental friendly and safe products by using the State-of-the-Art platform called Oryz^HiExp, having high capacity to express proteins or small peptides in the rice grain. The recombinant fibronectin (OsrhFN) was expressed in the rice endosperm platform using the world's top purification processing. The OsrhFN is animal component free and has good batch consistency, and has been demonstrated that has the same physical and chemical with the plasma derived FN, while has higher purity and bioactivity. The OsrhFN can support stem cells growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions.

OsrhFN is a chemical defined, xeno-free cell culture matrix that supports the growth and differentiation of stem cells under feeder-free conditions. The xeno-free cell culture system allows complete control over the culture environment, resulting in more consistent cell populations and reproducible results in downstream applications. Healthgen biotech rice endosperm platform makes the manufacture capability at multi-gram scales to meet your bioprocessing needs, which also can keep batch consistency and can provide stable supply.