Oryzogen Recombinant Fibronectin Enhance Cell Culture Performance 206kD

Recombinant fibronectin enhance cell culture performance

Description

Fibronectin (FN) is a particularly important and well-studied component of the extracellular matrix, and is known to play a key role in cell adhesion, spreading, migration, differentiation and proliferation via interactions with transmembrane integrin receptors. FN is a 220 kDa glycoprotein composed of 2 subunits bound via a disulfide bond, and exists as a soluble circulating protein in body fluids like plasma, but can be rearranged to a fibrillar insoluble component in the extracellular matrix of cells. FN possesses anacidic isoelectric point (pI = 5.5–6.0), and so is negatively charged at a physiological pH (7.4).

FN molecule contains several functionally and structurally distinct domains which may bind to cell surfaces, collagen, fibrinogen or fibrin, complement, glycosaminoglycans, proteoglycans and heparin. FN can enhance cell adhesion and spreading and affect the routes of cell migration in cell culture, which is quite of importance for the cell growth and proliferation. FN is suitable as a thin coating on cell/tissue culture surface for a variety of cell types, which can also be used as an additive to serum –free culture medium.

Healthgen Biotech used the rice endosperm cells as a bioreactor to specifically and highly express the recombinant human fibronectin (OsrhFN), which is non-animal component and has excellent batch consistency and high purity. The OsrhFN has been demonstrated that has the same physical and chemical with the plasma derived FN, while has higher purity and bioactivity.

In the CHO-K1 cell culture, the different concentration of OsrhFN was tested, which result showed that the OsrhFN can significantly promote cell adhesion and enhance cell performance.

The recombinant human fibronectin (OsrhFN) derived from rice endosperm platform has such advantages:

Scalable: Rice endosperm platform makes the manufacture capability at multi-gram scales to meet your bioprocessing needs.

Cost effective: Providing consistent performance with lower cost and time consuming, especially in bioprocessing.

Stable supply: The rice endosperm platform can keep batch consistency and can provide stable supply.

Non-animal component: Free of animal or human source, avoid any potential risks of animal, bacteria, prions, virus and contagious protein.

High Bioactivity: The expressed protein can stored in the protein body in rice grain, which can keep the protein bioactivity, prevents the changes of physical, chemical properties and biological characteristics.

Easy downstream purification: The well-defined component of cell culture medium make the downstream purification and research easily.