220 KDa High Purity Recombinant Fibronectin FN For Cell Culture Medium HYC021M01

High purity recombinant fibronectin for cell culture medium

Description

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfidebound polypeptides of molecular weight 220 kDa. Functional domains of FN mediate interactions with cell surface receptors and a number of other macromolecules including fibrin, fibrinogen, collagen, heparin, immune complexes, and fibronectin itself. Numerous studies have shown that FN can enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture. FN has been shown to also play a role in cellular morphology, cytoskeletal organization, phagocytosis, hemostasis, embryonic differentiation and wound repair. FN is suitable as a thin coating on cell/tissue culture surface for a variety of cell types.

Currently, the FN is purified from the plasma, which however is limited by the availability of supply. In addition, due to shortage of plasma and low expression levels of recombinant fibronectin in conventional gene expression systems, Healthgen Biotech express the recombinant fibronectin (OsrhFN) in the rice endosperm platform using the world's top purification processing. The OsrhFN is animal component free and has high purity, and has been demonstrated that has the same physical and chemical with the plasma derived FN. The OsrhFN can support cells growth, enhance cell adhesion, spreading and stem cells differentiation in serum –free culture medium.

The OsrhFN is derived from the rice endosperm, which has high purity and bioactivity, provides a safety solution to replace the plasma derived FN.

Physical Appearance: White lyophilized powder

Purity: More than 95% as determined by SDS-PAGE.

Storage condition: Stored in -20℃

In the SDS-PAGE and SEC-HPLC purity analysis, the purity of OsrhFN is more than 95%.

OsrhFN is a chemical defined, xeno-free cell culture matrix that supports the cell growth and enhances cell adhesion and spreading. Its advantages are:

Scalable: Rice endosperm platform makes the manufacture capability at multi-gram scales to meet your bioprocessing needs.

Stability and Activity in Culture: Providing consistent performance in cell culture media with lower cost and time consuming, especially in bioprocessing.

Non-animal component: Free of animal or human source, avoid any potential risks of animal, bacteria, prions, virus and contagious protein.

Easy downstream purification: The well-defined component of cell culture medium make the downstream purification and research easily.