ISO Recombinant Protein Service Rice Endosperm Specific Expression Platform

ISO Recombinant Protein Service Rice Endosperm Specific Expression Platform​

Rice endosperm cell-specific and efficient expression technology platform for proteins and polypeptide expression of technical services, to achieve a stable and efficient expression of the purpose of protein gene engineering rice lines, can also provide the purpose of different levels of protein and peptide purification process development.

• Provide the rice endosperm expression technology to expression customized proteins

• Target protein expression (100~1000 amino acids)
• Small molecular polypeptides (less than 100 amino acids)
• Expression level reaches 0.1~0.9% of brown rice dry weight

• Purification Process Development

establish easy and efficient, low cost of extraction, purification process, and provide technical solutions for any problems in the scale production.

Common Equipment 

Lyophilization Unit

The lyophilization unit, in Class 100 clean area, is composed of two sets of lyophilizers, and aseptic transferring window, hot and damp heat sterilization cabinet, and manual packing machine, etc. The products are prepared and packed in the sterile area after lyophilization. The production capacity reached 4-5kg lyophilization powder per batch.

Ultrafiltration Unit

The ultrafiltration unit is composed of an ultrafiltration system and peristaltic pumps in Class C+A clean area. The Millipore manual ultrafiltration fixture, which can be installed with 2.5-5.0 square meter membrane, can be applied to various sizes of membrane pore size and ultrafiltration system. The ultrafiltration system is in the Class A clean area, which provides good security for aseptic operation.

Chromatographic Purification Unit

The chromatographic purification unit, in the Class C clean area in accordance with GMP, is composed of 50~1000L buffer solution tanks, chromaflow 800 columns, peristaltic pumps, industrial-grade UV detectors, spectrum acquisition systems, and so on. The system also can match with other chromatography columns diameter from 100-800mm, such as BPG series (GE Healthcare), VERSA 450, and so on. The column packing and unpacking are performed by automatical Master Customer Asm (GE Healthcare), which also matched a series of chromatography resins, such as ion-exchange chromatography, hydrophobic chromatography, and so on.

Extraction Unit

The extraction unit, in the Class D clean area, is composed of 2 sets of 1000L buffer preparation tanks, 3 sets of 1000L extracting tanks, a pipeline system, a sanitary centrifugal pump, a frame filter press, and a cartridge filter. The buffer preparation tank can actualize buffer automatic mixing, heating and cooling, feed fluid transportation. The frame filter press is equipped with 50~300um aperture, and microporous filter, in 5 or 10 inches, can be matched with 0.22um, 0.45um, and 1um filter element and so on. All tanks are washed by pure water and injection water spraying and sterilized by pure steam.