Greater Than 95% Purity bFGF Growth Factor Keeps Stem Cells In Undifferentiation 106096-93-9

Greater Than 95% Purity bFGF Growth Factor Keeps Stem Cells In Undifferentiation

Description

Wuhan Healthgen Biotechnology Corp. is based on the State-of-the-art platform called Oryz^HiExp. As a eukaryotic, rice has stable genetic characteristics. It can be ensured the bFGF bioactivity and stability in rice endosperm cells. It fundamentally ensures the high batches stability. In the two lots bFGF bioactivity test result, the EC50 of both two are less than 0.1, which means that both of these two lots bFGF have high bioactivity. And in the ESC and iPS cell culture, bFGF was effective for keeping pulripotency from the morphology of colonies and Immunofluorescence staining.

Quick Details

1. CAS No.: 106096-93-9
2. Other Names: Plant-derived bFGF, OsrhbFGF, rbFGF, recombinant bFGF, bFGF, FGF2, FGF-β
3. Purity: Greater than 95% as determined by SDS-PAGE.
4. Source: Rice Grain (Oryza Sativa)
5. Formulation: OsrhbFGF is lyophilized with OsrHSA as the stabilizer.
6. Endotoxin: Less than 1EU/ ug
7. Biological Activity: The ED₅₀ is ≤1ng/ml determined by a cell proliferation assay using
8. Restrictions: FOR RESEARCH, LABORATORY AND MANUFACTURE USE ONLY

Advantages

1. The OsrhbFGF was expressed in the rice grain, then extracted and purified from it by patented technology. In the processing, the OsrhbFGF was storage in the rice endosperm protein bodies, which result in keep the bioactivity and stability. And the process does not involve the chemical reaction, which is a great extent to ensure the bioactivity of OsrhbFGF using the Balb/c 3T3 model to test the OsrhbFGF bioactivity, it is much higher than the E.coli derived bFGF.

2. As an expression platform with rice endosperm cell as the carrier, it has a great advantage compared with the bacteria bioreactor. Firstly, rice is a self – pollination crop, greatly guarantee the genetic stability; Secondly, there is almost sterile condition in the endosperm cells, which is easy to purify it and avoid some bacteria themselves impurities. Thereby, Healthgen OsrhbFGF has less endotoxin level, which is less than 1EU/ug.

Supplemental Description

Keep the mind, do not forget the original intention. Retaining the spirit of green, safe and healthy, Healthgen Biotechnology Corp. has constantly implemented this policy. For ten years, we have been strict to the most with every work we have done and pursue the highest quality. Our company is equipped with 1,500 square meters’ purification plant, including D-class, C-class and B + A-class clean area, with the production precess of liquid dosing, extraction, filtration clarification, evaporation, extraction , subpackage and freeze-drying. The plant has two Millipore CUF 100 evergreen systems, five GE AxiChrom 1000mm industrial grade columns and five AKTAprocess industrial grade chromatography systems, can achieve a highly purified protein production, ensuring the OsrhbFGF is really high purity.