Oryzogen Recombinant Lysozyme Powder From Rice Platform Bulk Supply CAS No 9001-63-2

Recombinant lysozyme from rice platform bulk supply

Description

Lysozyme is a potent antimicrobial enzyme, which has been extensively studied in birds, mammals, plants, insects, and bacteria. Lysozyme was shown to have bactericidal activity against M. lysodeikticus, a fact that remains used today when examining lysozyme activity. Lysozyme kills bacteria by catalytic hydrolysis of their cell wall peptidoglycan, which contains sugars and amino acids. The sugar component consists of A-(1,4)-linked N-acetylglucosamine and N-acetylmuramic acid and this bond is broken by lysozyme. This compromises the bacterial cell wall and causes the bacterium to burst under the high internal osmotic pressure. Lysozyme has two major domains, in addition to one active site. In lysozyme’s amino acid chain, glutamine and aspartic acid are two amino acids that are critical to the activity of this enzyme. Lysozyme is a small protein with 14.3 kDa molecular weight and a pI (isoelectric point) of 11.4 (making it highly positively charged at neutral pH), and contains 129 amino acids. Lysozyme (LYZ) is an antimicrobial enzyme produced by animals that forms part of the innate immune system.

Currently, the commercial lysozyme is mainly produced from milk, which however is limited by the availability of supply and source. Healthgen Biotech used the rice endosperm cells as a bioreactor to specifically and highly express the recombinant human lysozyme (OsrhLYZ), which is non-animal component and has excellent batch consistency and high purity. The rice endosperm expression platform advantages are over other systems in terms of scalability, safety, and cost-effectiveness. In addition, the rice endosperm provides the ideal place in which the seed is not only providing a stable site for the deposition of recombinant proteins to prevent from the degradation by proteases, but also storing at ambient temperatures for several years.

The OsrhLYZ has been demonstrated that has the same physical and chemical with the plasma derived lysozyme, while has higher purity and bioactivity. The OsrhLYZ purity is higher than 90% and bioactivity is more than 4X10^4 IU/mg. The OsrhLYZ has been demonstrated that has the same physical and chemical with the native lysozyme.

The purity of OsrhLYZ was tested via SEC-HPLC, which indicated that the purity is more than 90%.

The rice endosperm platform has such key benefits:

• Scalable: Rice endosperm platform makes the manufacture capability at multi-gram scales to meet your bioprocessing needs.
• Cost effective: Providing consistent performance with lower cost and time consuming, especially in bioprocessing.
• Stable supply: The rice endosperm platform can keep batch consistency and can provide stable supply
• Non-animal component: Free of animal or human source, avoid any potential risks of animal, bacteria, prions, virus and contagious protein.
• High Bioactivity: The expressed protein can stored in the protein body in rice grain, which can keep the protein bioactivity, prevents the changes of physical, chemical properties and biological characteristics.