Oryza Sativa Recombinant HSA Expressed From Rice Endosperm Specific Platform

Recombinant Human Serum Albumin HSA Expressed From Rice Endosperm Specific Platform​

Quick detail

1. CAS No.: 70024-90-7
2. Other Names: Recombinant HSA, rHSA, OsrHSA
3. EINECS No.: 274-272-6
4. Amino Acids Sequence: Same as the HSA from plasma
5. N-terminus: DAHKSEV
6. C-terminus: KLVAASQAALGL
7. Glycosylation: No
8. PI: 4.8
9. Thermal Stability: M/P 65℃
10. Lipid Conjugation: Same as the HSA from plasma
11. Crystal Structure: Same as the HSA from plasma
12. Storage and Handling: Stored at temperatures 2-8℃ for 48 months

Description

Healthgen Biotech uses State-of-the-art platform called Oryz^HiExp, having high capacity to express proteins and peptides in the rice grain. The platform uses rice endosperm cells as a bioreactor to specifically and highly express various recombinant proteins and small peptides. The platform has such advantages, free of animal prions or virus from human plasma, more biological safety, environment friendly compared with other bioreactors. The rice derived rHSA is animal component free and can be used in the cell culture media supplements, cosmetics additivie, industrial biological reagents in pharmaceuticals processing and life science research.

Advantages

1. Healthgen Biotech recombinant human serum albumin (rHSA) has excellent batch consistency. Using self-pollination rice as the bioreactor to express the rHSA, with the world's advanced facility and top production proceeding to extract and purify the rHSA, Healthgen Biotech can ensure the excellent consistency of rHSA from different batches.

2. The rice endosperm specific expression platform is easy to be large-scale. The rice endosperm has the protein body, which can keeps proteins stability and bioactivity.

At present, the production capacity is almost 1000 kg / year.

3. Recombinant human serum albumin (rHSA) has good efficacy, such as rHSA has the same second and tertiary structure, physiological and biochemical properties with HSA from plasma. In addition, compared with HSA from plasma, rHSA has a lower immune source stimulation, and plays a better role in accelerating cell growth and metabolism, because rHSA is animal component free, which can avoid the prions or virus contaminants.

Supplemental Description

The research results have been granted with 16 international patents, covering countries, such as United States, Japan, Europe, Canada and South Africa. Parts of the patents are showed as below:

A callus-specific promoter of rice and the use thereof

China patent no. 201110054109.X

Method for expressing small peptides using cereal non-storage proteins as fusion carrier in endosperm and the use thereof

US patent no. US20070289033

Japan patent no. JP2007-325594A

Europe patent no. EP1865064

Canada patent no. CA2587092

China patent no. 200610019285.9

A method for extracting recombinant human serum albumin from transgenic rice grain

South Africa patent no. 2013/04359

China patent no. 201010597544.2

A method for isolating and purifying recombinant human serum albumin from transgenic rice grain;

South Africa patent no. 2013/04358

China patent no. 201010606635.8

A method for isolating and purifying recombinant human serum albumin from transgenic rice grain

South Africa patent no. 2013/04358

US patent no. US9023990 B2

Production of recombinant human serum albumin with rice endosperm cells as biological reactor

China patent no. ZL 2005 1 0019084.4

Based on the innovative technology, Healthgen Biotech also published research papers.

Publications

1. He Y, et al. Large-scale production of functional human serum albumin from transgenic rice seeds. Proc Natl Acad Sci USA. 2011;108:19078-19083.

2. Na An, et al. Expression of a Functional Recombinant Human Basic fibroblast Growth Factor from Transgenic Rice Seeds. Int. J. Mol. Sci. 2013, 14, 3556-3567

3. Liping Z, et al. Expression and characterization of recombinant human alpha-antitrypsin in transgenic rice seed. Journal of Biotechnology. 2012; 164: 300-308.

4. Xie, T, et al. A biologically active rhIGF-1 fusion accumulated in transgenic rice seeds can reduce blood glucose in diabetic mice via oral delivery. Peptides 2008: 29: 1862-1870.

5. Zhen Chen, et al. Human serum albumin from recombinant DNA technology: challenges and strategies. Biochimica et Biophysica Acta. 2013; 1830: 5515-5525.

6. Zhen Chen, et al. Quantitation of the residual DNA from rice-derived recombinant human serum albumin. Analytical Biochemistry. 2014; 450: 4-10.

7. Jiquan Ou, et al. Transgenic rice endosperm as a bioreactor for molecular pharming. Plant Cell Rep (2014) 33:585–594

8. Kai fu, et al. Immunotoxicity assessment of rice-derived recombinant human serum albumin using human peripheral blood mononuclear cells. PLoS ONE 01/2014; 9(8): e104426.